But documents seen by The Age and the Sydney Morning Herald show the nurse and police officer returned positive saliva swabs over the weekend.
The Department of Health and Human Service’s outbreak squad visited the hotel on Sunday and areas where the nurse and police officer worked have been cleaned.
The Alfred Health nurse worked in a “front of house” role at the hotel and was responsible for checking temperatures on those arriving there. She wore full personal protective equipment and a face shield.
The Victoria Police officer worked as a floor monitor.
DHHS contact tracers are investigating where both have travelled outside of work in the past week.
Alfred Health has stood down 36 staff at the hotel, including 33 nurses and three cleaning staff.
At least four other Victoria Police members have been put into isolation after dining with the officer who returned the positive saliva swab.
The Holiday Inn is being used as a “hot” hotel to quarantine COVID-19 positive returning travellers.
Transmission of the virus between returning travellers and private security guards in May and June last year sparked Victoria’s second wave of COVID-19, causing hundreds of deaths and months of hard lockdowns.
DHHS and Victoria Police have been contacted for comment.